DEAE and CM Bio-Gel® A Ion Exchange Gels Instruction Manual

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DEAE-Cellulose is a cellulose ion-exchange adsorbent used in ion exchange chromatography and to separate lipids found in plasma membrane microdomain sites  DEAE cellulose 52 is a preswollen weak anion exchange resin provides high binding capacity and clean resolution

jagananna tab reset 2024 column of beads containing carboxylate groups, and can then chromatography on positively charged diethylaminoethyl-cellulose columns  How do i decide on the pH of the column buffer if protein pI is unknown? Does DEAE-cellulose require activation or would it remain positive at neutral pH?