Automation of CyQuant LDH Cytotoxicity Assay using Biomek i7

Publication protocol Cell migration was measured using Corning transwell inserts with μm pore polycarbonate membrane

Automation of CyQuant LDH Cytotoxicity Assay using Biomek i7 CyQuant® GR Dye : One 25 µL tube 4 Cotton Swabs (Part No described in Assay Protocol References 1 Ridley

Publication protocol Cell migration was measured using Corning transwell inserts with μm pore polycarbonate membrane

cyquant protocol This protocol uses DMSO as a solubilizing agent for formazan to shorten the time of the assay Replace the culture medium

The assay employs CyQUANT GR dye, which produces a large fluorescence enhancement upon binding to cellular nucleic acids that can be measured using standard

cyquant protocol CyQuant® DNA assays in 96 protocol by blotting on laboratory wipes, and stored at −20 °C

Cell proliferation was assessed by measuring fluorescence following 120 hours of indicated compound treatment using the CyQUANT Cell